Our new Very clear+ tissue clearing strategy is the one process that delipidates samples with no alter in morphology and with minimum effect on structural integrity.

Megatome is often a vibrating microtome meant to section a broad selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With large blade vibrating frequency and minimized blade deflection, Megatome permits superior-throughput tissue sectioning with uniform area profile, and nominal tissue problems and knowledge decline.

Antibodies may well acquire months to diffuse by way of just a few millimeters of tissue, that has a steep labeling gradient from floor to Main.

Operating the CLI directly from a Gradle job is not currently supported. A distribution has to be developed by means of gradlew :j2d-cli:distZip to create a zip file that contains anything necessary to operate.

SE makes use of a rotational electrical area to disperse highly electromobile molecules (such as antibodies or surfactant micelles) all over a porous sample devoid of detrimental electrically charged structures within the tissue. This allows two-4 day clearing of intact organs,

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Megatome is a novel microtome that enables for high-precision sectioning of a variety of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with minimal tissue problems and information decline.

eFLASH is actually a rapid tissue labeling procedure that permits for uniform full-organ staining in 20 rounds of labeling.

The SmartSPIM light-weight sheet microscope gives unparalleled resolution and acquisition speed all through intact tissue volumes, although its modular design lets you upgrade and personalize individual parts.

Docset creation calls for at minimum two possibilities: the name in the docset and The situation in the Javadoc data files to include while in the docset.

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SHIELD avoids the variability of hydrogel embedding and the knowledge decline from PFA preservation, guarding specimens for a number of rounds of processing.

Leverage the Distinct+ tissue clearing method, coupled with eFLASH and patented stochastic electrotransport technologies, to rapidly distinct and label complete organs. Critical highlights and options include:

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